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sterile 10 st btl  (Greiner Bio)


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    Greiner Bio sterile 10 st btl
    Sterile 10 St Btl, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sterile+petri+dishes/Petri+Dish+Square+Ps+Clear/pmc12925450-62-7-10
    Average 96 stars, based on 624 article reviews
    sterile 10 st btl - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Differences of in vitro immune responses between patent and pre-patent Litomosoides sigmodontis –infected mice are independent of the filarial antigenic stimulus used
    Article Snippet: .. Adult worms were isolated from the TC of infected jirds (d260 p.i.), transferred into sterile Petri dishes (Greiner GmbH, Frickenhausen, Germany), and rinsed several times with endotoxin-free sterile PBS (PAA, Linz, Austria). ..

    Infection:

    Article Title: Differences of in vitro immune responses between patent and pre-patent Litomosoides sigmodontis –infected mice are independent of the filarial antigenic stimulus used
    Article Snippet: .. Adult worms were isolated from the TC of infected jirds (d260 p.i.), transferred into sterile Petri dishes (Greiner GmbH, Frickenhausen, Germany), and rinsed several times with endotoxin-free sterile PBS (PAA, Linz, Austria). ..

    Sterility:

    Article Title: Differences of in vitro immune responses between patent and pre-patent Litomosoides sigmodontis –infected mice are independent of the filarial antigenic stimulus used
    Article Snippet: .. Adult worms were isolated from the TC of infected jirds (d260 p.i.), transferred into sterile Petri dishes (Greiner GmbH, Frickenhausen, Germany), and rinsed several times with endotoxin-free sterile PBS (PAA, Linz, Austria). ..

    Article Title: Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum
    Article Snippet: .. Col-0, pHSP70::LUC [ ], pICE1::LUC and pPIF4:PIF4-LUC [ ] seeds were gas-sterilized by chlorine gas for 3 h. The sterile seeds were sown on sterile Petri dishes (Greiner Bio-One, 688,161) containing 0.8% plant agar, full strength Murashige-Skoog medium as indicated above, without sucrose. ..

    Article Title: Protocol for a minigene splice assay using the pET01 vector
    Article Snippet: PCR SingleCap 8-strip tubes, 0.2 mL , Biozym , Cat# 710988. .. Sterile petri dishes, 94 × 16 mm , Greiner , Cat# 633181. .. Parafilm M sealing film , Merck , Cat# HS234526B.

    Article Title: Is a Combination of Metals More Toxic to Mosses Than a Single Metal?
    Article Snippet: .. For the control, the medium nutrients and agar were added to distilled water, mixed, autoclaved, and poured into sterile Petri dishes (6 cm, Greiner bio-one, Kremsmünster, Austria) on a sterile bench. ..

    Article Title: Listeria monocytogenes uses de novo purine synthesis to enhance fitness in Lyoner-type sausage
    Article Snippet: .. Two hundred microliters of the main culture were inoculated onto a slice of Lyoner (10 g, diameter of each slice was 9,3 cm), placed in sterile petri dishes (633,180, Greiner BioOne, Germany), and incubated at 8 ◦C for 48 h. Quantification was performed on oxford agar (Merck KGaA) plates that were incubated at 37 ◦C for 24 h. These larger volume screenings were repeated three times. ..

    Article Title: Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum.
    Article Snippet: .. Col-0, pHSP70::LUC [31], pICE1::LUC and pPIF4:PIF4LUC [62] seeds were gas-sterilized by chlorine gas for 3 h. The sterile seeds were sown on sterile Petri dishes (Greiner Bio-One, 688,161) containing 0.8% plant agar, full strength Murashige-Skoog medium as indicated above, without sucrose. ..

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Article Title: Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum
    Article Snippet: .. The sterile seeds were sown on square sterile Petri dishes (Greiner Bio-One, 688161) containing 0.8% plant agar, full strength Murashige-Skoog (MS including MES Buffer and vitamins, Duchefa) medium without sucrose. ..

    Control:

    Article Title: Is a Combination of Metals More Toxic to Mosses Than a Single Metal?
    Article Snippet: .. For the control, the medium nutrients and agar were added to distilled water, mixed, autoclaved, and poured into sterile Petri dishes (6 cm, Greiner bio-one, Kremsmünster, Austria) on a sterile bench. ..

    Incubation:

    Article Title: Listeria monocytogenes uses de novo purine synthesis to enhance fitness in Lyoner-type sausage
    Article Snippet: .. Two hundred microliters of the main culture were inoculated onto a slice of Lyoner (10 g, diameter of each slice was 9,3 cm), placed in sterile petri dishes (633,180, Greiner BioOne, Germany), and incubated at 8 ◦C for 48 h. Quantification was performed on oxford agar (Merck KGaA) plates that were incubated at 37 ◦C for 24 h. These larger volume screenings were repeated three times. ..

    Recombinant:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Plasmid Preparation:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Software:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Polymerase Chain Reaction:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Blocking Assay:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Concentration Assay:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Modification:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Selection:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..

    Marker:

    Article Title: Protocol for a minigene splice assay using the pET01 vector.
    Article Snippet: .. Continued REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA Plasmid: Exontrap vector pET01 MoBiTec GmbH Cat# PET01 Software and algorithms ApE, A plasmid Editor Davis and Jorgensen 5 https://jorgensen.biology.utah.edu/ wayned/ape/ Alamut Visual Plus Sophia Genetics https://www.sophiagenetics.com/sophiaddm-for-genomics/alamut-visual-plus/ Ensembl Harrison et al. 6 https://www.ensembl.org/index.html UCSC Genome Browser Raney et al. 7 https://genome.ucsc.edu/cgi-bin/hgBlat Primer3 Untergasser et al. 8 https://primer3.ut.ee/ NEBaseChanger New England Biolabs https://nebasechanger.neb.com/ T m calculator New England Biolabs https://tmcalculator.neb.com/#!/main T m for Oligos Calculator Promega https://www.promega.de/resources/ tools/biomath/tm-calculator/ SNPCheck EMQN and Certus Technology https://genetools.org/SNPCheck/ snpcheck.htm GeneMapper Software 5 Applied Biosystems https://www.thermofisher.com/order/ catalog/product/4370784 Other Arium Pro ultrapure water system (refered to as H 2 O) Sartorius Cat# H2OPRO-UV-T Heracell 150 CO 2 incubator Thermo Fisher Scientific N/A 6-well CytoOne plate, TC-treated Starlab Cat# CC7682-7506 Screw cap tube, 50 mL SARSTEDT Cat# 62.547.254 SafeSeal reaction tube, 1.5 mL, PP, PCR performance tested (referred to as Eppi in this protocol) SARSTEDT Cat# 72.706,400 PCR SingleCap 8-strip tubes, 0.2 mL Biozym Cat# 710988 Sterile petri dishes, 94 3 16 mm Greiner Cat# 633181 Parafilm M sealing film Merck Cat# HS234526B Fresco 21 microcentrifuge Thermo Fisher Scientific Cat# 75002425 S1000 thermal cycler with dual 48/48 fast reaction module Bio-Rad Cat# 1852148 Gel documentation system NIPPON Genetics EUROPE N/A NanoDrop One C Thermo Fisher Scientific Cat# ND-ONEC-W Thermomixer comfort heat block Eppendorf Cat# 5355 000.011 3730xl DNA analyzer Applied Biosystems Cat#A41046 Supplemented RPMI/DMEM medium Reagent Final concentration Amount RPMI 1640 medium or Dulbecco’s Modified Eagle medium, high glucose, pyruvate – 500 mL Penicillin-Streptomycin (10,000 U/mL) ∼90 U/mL 5 mL FBS Superior ∼9% 50 mL Amphotericin B (250 μg/mL) ∼700 ng/mL 1.6 mL Total N/A 556.6 mL LB agar plates with selection marker (50 μg/mL) Reagent Amount Tryptone/Peptone ex casein pancreatically digested 3 g Sodium chloride 3 g (Continued on next page) STAR Protocols 6, 103908, September 19, 2025 5 Note: Autoclave the LB agar mixture and let it cool down to ∼60 ◦ C before adding the antibiotics. ..



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